mathstodon.xyz is one of the many independent Mastodon servers you can use to participate in the fediverse.
A Mastodon instance for maths people. We have LaTeX rendering in the web interface!

Server stats:

2.7K
active users

#VirtualFlyBrain

0 posts0 participants0 posts today

Sometimes one stops to just look at the data. And the software user interface. They are beautiful.

We are looking at a cross section of the larval brain, near the brain commissure, where hundreds of neurons (magenta: their reconstructed skeletons) cross from one brain hemisphere to the other. To the right, a 3D rendering of multiple neurons, a pair of which cross the midline in a U-shaped bend.

Our CATMAID software is web-based, in other words it's just a website that accesses remote data. I credit it's sleek design to @herrsaalfeld – author of the early, "Ice Age" CATMAID and its blue tones – who at some point in his life studied "medieninformatik" and has always had a penchant for art.

See our images and fly neurons here, kindly hosted by the :
l1em.catmaid.virtualflybrain.o

Replied in thread

@CurrentBiology

Andreas Schoofs and Anton Miroschnikow in Michael Pankratz' lab have done an astonishing amount of work manually mapping the peripheral nervous system of the larval , with , in the STEM volume we imaged and they named "Igor". Congrats on seeing this gargantuan project through!

The whole larval volume of "Igor", including all tissues, is available here, courtesy of the project led by Aubrey Weigel @avweigel at :
openorganelle.janelia.org/data

See it at 5x5x35 nm resolution in : neuroglancer-demo.appspot.com/ (control-minus and control-plus to zoom; mouse click to pan, scroll wheel to browse in Z).

Will appear at the website soon as well.

There are many more peripheral nervous system components mapped, yet to be published.

This paper is open access:
"Serotonergic modulation of swallowing in a complete fly vagus nerve connectome", Schoofs et al. 2024 cell.com/current-biology/fullt

Today the peer-reviewed version of our preprint is out:

"The of an insect brain"
science.org/doi/10.1126/scienc

Congrats to co-first authors Michael Winding and Benjamin Pedigo, and to all our lab members and collaborators who made this work possible over the years. A journey that started over 10 years ago–and yet this is but a new beginning. So much more to come.

See my on the preprint from back in the Autumn: mathstodon.xyz/@albertcardona/

The data is available both as supplements and directly via thanks to hosting by the :
l1em.catmaid.virtualflybrain.o)

(The "Winding, Pedigo et al. 2023" annotation listing all included neurons will appear very soon in an upcoming update.)

Replied in thread

@dantracey @kristinmbranson @annikabarber @debivort @giorgiogilestro

Find the original basin-1 neurons at the at "Tools - CATMAID - Hosted EM Data - Larval - Larva (ABD1.5)" which opens a server abd1.5.catmaid.virtualflybrain)

Find them via Neuron Search (icon with a "?").

The "Construction time" is wrong (see "Summary info" of the Selection Table) because these neurons were imported from . Old enough to predate the software!

Continued thread

The web-based open source software was devised as "google maps but for volumes". Documentation at catmaid.org and source code at github.com/catmaid/CATMAID/

Modern enables hundreds of researchers world wide to collaboratively map neuronal circuits in large datasets limited only by bandwidth and server-side storage to map and analyse a whole brain .

See the larval CNS at the server: l1em.catmaid.virtualflybrain.o)

Replied in thread

@manlius Yes, a lot, but generated mostly with #CATMAID which is more purpose-built for #connectomics.

An early reconstruction of a neural circuit done with #TrakEM2 was by Davi Bock et al. 2011 on the mouse visual cortex, "Network anatomy and in vivo physiology of visual cortical neurons" nature.com/articles/nature0980

Another one with #TrakEM2 was by Dan Bumbarger et al. 2013 "System-wide rewiring underlies behavioral differences in predatory and bacterial-feeding nematodes" where they compared #celegans with another nematode, #pristionchus pacificus that has the exact same amount of neurons but connected differently sciencedirect.com/science/arti

Later ones with #CATMAID include:

The polychaete worm #Platynereis by @jekely 's group, "Whole-animal #connectome and cell-type complement of the three-segmented Platynereis dumerilii larva" Verazto et al. 2020 biorxiv.org/content/10.1101/20

And all of ours in #Drosophila larva. See the #VirtualFlyBrain server which hosts the #vEM of the whole central nervous system and lists all the neurons included in each published paper (currently 23), shared among the papers and all connecting to each other: l1em.catmaid.virtualflybrain.o)

The 24th will come soon, featuring the complete whole #Drosophila larval brain with ~2,500 neurons. It's under review.

NatureNetwork anatomy and in vivo physiology of visual cortical neurons - NatureTo date, various aspects of connectivity have been inferred from electron microscopy (EM) of synaptic contacts, light microscopy of axonal and dendritic arbors, and correlations in activity. However, until now it has not been possible to relate the complex structural wiring between neurons to the function of individual cells. Using a combination of functional imaging and three-dimensional serial EM reconstruction at unprecedented scale, two papers now describe the connectivity of single cells in the mouse visual system. This study investigates the connectivity of inhibitory interneurons in primary visual cortex.

Have you visited the #FlyWire website yet? Both for helping proofread and analyze the whole #Drosophila brain #connectome, or simply to admire the beautiful renderings of neuronal arbors: join.flywire.ai

(See also the #VirtualFlyBrain for #ontology-driven navigation of the fly brain, and access to images of genetic driver lines, and more: v2.virtualflybrain.org/org.gep )

Wish I had time or resources to create such a beautiful landing page for the larval central nervous system. The #connectome of the whole larval brain is coming soon. For now, see the #vEM images and some ~3,000 published neurons in this #CATMAID server: l1em.catmaid.virtualflybrain.o)

Continued thread

What can you do with a #CATMAID server? Say, let's look at the #Drosophila (vinegar fly, often referred to as fruit fly) larval central nervous system, generously hosted by the #VirtualFlyBrain l1em.catmaid.virtualflybrain.o) or the #Platynereis (a marine annelid) server from the Jekely lab catmaid.jekelylab.ex.ac.uk/

First, directly interact by point-and-click: open widgets, find neurons by name or annotations, fire up a graph widget and rearrange neurons to make a neat synaptic connectivity diagram, or an adjacency matrix, or look at neuron anatomy in 3D. Most text–names, numbers–are clickable and filterable in some way, such as regular expressions.

Second, interact from other software. Head to r-catmaid natverse.org/rcatmaid/ (part of the #natverse suite by Philipp Schlegel @uni_matrix, Alex Bates and others) for an R-based solution from the Jefferis lab at the #MRCLMB. Includes tools such as #NBLAST for anatomical comparisons of neurons (see paper by Marta Costa et al. 2016 sciencedirect.com/science/arti ).

If R is not your favourite, then how about #python: the #navis package, again by the prolific @uni_matrix, makes it trivial, and works also within #Blender too for fancy 3D renderings and animations. An earlier, simpler version was #catpy by @csdashm github.com/ceesem/catpy , who also has examples on access from #matlab.

Third, directly from a #psql prompt. As in, why not? #SQL is quite a straightforward language. Of course, you'll need privileged access to the server, so this one is only for insiders. Similarly privileged is from an #ipython prompt initialized via #django from the command line, with the entire server-side API at your disposal for queries.

Fourth, and one of my favourites: from the #javascript console in the browser itself. There are a handful of examples here github.com/catmaid/CATMAID/wik but the possibilities are huge. Key utilities are the "fetchSkeletons" macro-like javascript function github.com/catmaid/CATMAID/wik and the NeuronNameService.getInstance().getName(<skeleton_id>) function.

Notice every #CATMAID server has its /apis/, e.g., at l1em.catmaid.virtualflybrain.o will list all GET or REST server access points. Reach to them as you please. See the documentation: catmaid.readthedocs.io/en/stab

In short: the data is there for you to reach out to, interactively or programmatically, and any fine mixture of the two as you see fit.

l1em.catmaid.virtualflybrain.orgCATMAID