Gaspar Jekely<p>The revised version of our <a href="https://biologists.social/tags/Platynereis" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Platynereis</span></a> <a href="https://biologists.social/tags/connectome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>connectome</span></a> paper is now out:</p><p><a href="https://elifesciences.org/reviewed-preprints/97964" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">elifesciences.org/reviewed-pre</span><span class="invisible">prints/97964</span></a></p><p>Cell-type-level annotation of the whole organisms, including synaptic and desmosomal connectomes. Can be explored with CATMAID here:<br><a href="https://catmaid-jekelylab.cos.uni-heidelberg.de" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">catmaid-jekelylab.cos.uni-heid</span><span class="invisible">elberg.de</span></a><br><a href="https://biologists.social/tags/larva" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>larva</span></a> <a href="https://biologists.social/tags/marine" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>marine</span></a> <a href="https://biologists.social/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>neuroscience</span></a> <a href="https://biologists.social/tags/vEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEM</span></a></p>
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Albert Cardona<p>GridTape TEM with beam deflection to reduce stage movements and increase imaging throughput:</p><p>"Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy", Zhao et al. 2024</p><p><a href="https://www.nature.com/articles/s41467-024-50846-4" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41467-024</span><span class="invisible">-50846-4</span></a></p><p><a href="https://mathstodon.xyz/tags/TEM" class="mention hashtag" rel="tag">#<span>TEM</span></a> <a href="https://mathstodon.xyz/tags/GridTapeTEM" class="mention hashtag" rel="tag">#<span>GridTapeTEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a> <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a> <a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/microscopy" class="mention hashtag" rel="tag">#<span>microscopy</span></a> <a href="https://mathstodon.xyz/tags/ElectronMicroscopy" class="mention hashtag" rel="tag">#<span>ElectronMicroscopy</span></a></p>
Albert Cardona<p><span class="h-card" translate="no"><a href="https://chaos.social/@nanographs" class="u-url mention">@<span>nanographs</span></a></span> </p><p>Thanks for sharing this – will have to think about it. In our case, we built in-house the gridtape reel holders which amount to a custom stage with a mini-camera in it for identifying slots and controlling imaging. So we don't use the factory-supplied stage.</p><p><a href="https://www.biorxiv.org/content/10.1101/657346v1" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/65</span><span class="invisible">7346v1</span></a></p><p><a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/volumeEM" class="mention hashtag" rel="tag">#<span>volumeEM</span></a> <a href="https://mathstodon.xyz/tags/TEM" class="mention hashtag" rel="tag">#<span>TEM</span></a></p>
Albert Cardona<p>True as always that the way to make software run faster is to make it do less operations. After all, CPUs can only execute a fixed number of operations per unit of time.</p><p>Here, I tweaked code for serial section registration that drops execution time from 27 seconds to 100 milliseconds: a 270x speed up.</p><p>All it had to do is to search for matching SIFT features in one image only within a predetermined radius centered on one SIFT feature in another image. Extremely effective for when e.g., the maximum translation is known.</p><p>The matching code using a KDTree:<br /><a href="https://github.com/acardona/scripts/blob/master/java/asm/my/PointMatchesFast.java#L56" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">github.com/acardona/scripts/bl</span><span class="invisible">ob/master/java/asm/my/PointMatchesFast.java#L56</span></a></p><p>The test script:<br /><a href="https://github.com/acardona/scripts/blob/dev/python/imagej/FIBSEM/tests/test_matchNearbyFeatures.py" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">github.com/acardona/scripts/bl</span><span class="invisible">ob/dev/python/imagej/FIBSEM/tests/test_matchNearbyFeatures.py</span></a></p><p><a href="https://mathstodon.xyz/tags/FijiSc" class="mention hashtag" rel="tag">#<span>FijiSc</span></a> <a href="https://mathstodon.xyz/tags/java" class="mention hashtag" rel="tag">#<span>java</span></a> <a href="https://mathstodon.xyz/tags/jython" class="mention hashtag" rel="tag">#<span>jython</span></a> <a href="https://mathstodon.xyz/tags/volumeEM" class="mention hashtag" rel="tag">#<span>volumeEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a></p>
Gaspar Jekely<p>Also check the nice supplementary movies of <a href="https://biologists.social/tags/vEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEM</span></a> reconstructions <br><a href="https://www.nature.com/articles/s41467-024-48044-3#Sec20" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41467-024</span><span class="invisible">-48044-3#Sec20</span></a></p>
Gaspar Jekely<p>New paper from Ikeda et al. on the biogenesis of chitin bristles in the annelid <a href="https://biologists.social/tags/Platynereis" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Platynereis</span></a> with nice <a href="https://biologists.social/tags/vEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEM</span></a> reconstructions and a chitin synthase knockout.<br>Bristles are formed in a process of biological 3D printing. <span class="h-card" translate="no"><a href="https://mander.xyz/c/biology" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@<span>biology</span></a></span> <br><a href="https://biologists.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> <br><a href="https://www.nature.com/articles/s41467-024-48044-3" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41467-024</span><span class="invisible">-48044-3</span></a></p>
Albert Cardona<p>From Moritz Helmstaedter on <a href="https://mathstodon.xyz/tags/RoboEM" class="mention hashtag" rel="tag">#<span>RoboEM</span></a>:</p><p>"In today’s AI research, any project lasting longer than 6 months is considered slow, if not outdated and overrun by history. RoboEM, with a concept and an approach that seem quite plausible, still took 5 years from idea to the fully evaluated tool reported here. This was only possible with the patient support of the Max Planck Society, which encourages long-term projects, and the tenacity of the first author. Details made all the difference. And, as often, dead ends had to be avoided efficiently. We had to abandon, for example, the idea of using steering variability to indicate branch points — a nice analogy to road intersections in car steering, but too far-fetched to work well enough in brain tissue data. Sometimes giving up beloved ideas is as important as following through on others. M.H."</p><p><a href="https://www.nature.com/articles/s41592-024-02227-4.epdf?sharing_token=nElv1SiMTpxjPsXqxX9xMtRgN0jAjWel9jnR3ZoTv0Nrov_SWdBALYS97F1eM5Lk_JEBGPmcigRE4GeE5PJKr0YlUmXLIqorYFzRm9BanPX-UNoWn8B-lFTNgSYQl_gdPFu85a_vT5pFp1FCyd6dRPVpDqcS14IBc1pS4_ue4ho%3D" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41592-024</span><span class="invisible">-02227-4.epdf?sharing_token=nElv1SiMTpxjPsXqxX9xMtRgN0jAjWel9jnR3ZoTv0Nrov_SWdBALYS97F1eM5Lk_JEBGPmcigRE4GeE5PJKr0YlUmXLIqorYFzRm9BanPX-UNoWn8B-lFTNgSYQl_gdPFu85a_vT5pFp1FCyd6dRPVpDqcS14IBc1pS4_ue4ho%3D</span></a></p><p><a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a> <a href="https://mathstodon.xyz/tags/volumeEM" class="mention hashtag" rel="tag">#<span>volumeEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/MaxPlanckSociety" class="mention hashtag" rel="tag">#<span>MaxPlanckSociety</span></a></p>
Albert Cardona<p>“Perception: How larvae feel the world around them” by Jimena Berni <a href="https://elifesciences.org/articles/96708" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">elifesciences.org/articles/967</span><span class="invisible">08</span></a></p><p>… an insight piece on Andreas Thum’s lab work on mapping the sensory organs of the <a href="https://mathstodon.xyz/tags/Drosophila" class="mention hashtag" rel="tag">#<span>Drosophila</span></a> larva with electron microscopy:</p><p>“Morphology and ultrastructure of external sense organs of Drosophila larvae”<br />Richter et al. 2024 <a href="https://elifesciences.org/articles/96708" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">elifesciences.org/articles/967</span><span class="invisible">08</span></a></p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a></p>
Albert Cardona<p>Christel Genoud is recruiting an electron microscopy specialist at the University of Lausanne:<br /><a href="https://wp.unil.ch/emf/?trk=feed-detail_main-feed-card-text" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">wp.unil.ch/emf/?trk=feed-detai</span><span class="invisible">l_main-feed-card-text</span></a></p><p>Job ad:<br /><a href="https://career5.successfactors.eu/career?career%5fns=job%5flisting&company=universitdP&navBarLevel=JOB%5fSEARCH&rcm%5fsite%5flocale=fr%5fFR&career_job_req_id=21524&selected_lang=fr_FR&jobAlertController_jobAlertId=&jobAlertController_jobAlertName=&browserTimeZone=Europe/Zurich&_s.crb=bYQhIgqn0MbzwdRaA%2baluxpeS0wy0UARIy5GFiKiaEU%3d" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">career5.successfactors.eu/care</span><span class="invisible">er?career%5fns=job%5flisting&company=universitdP&navBarLevel=JOB%5fSEARCH&rcm%5fsite%5flocale=fr%5fFR&career_job_req_id=21524&selected_lang=fr_FR&jobAlertController_jobAlertId=&jobAlertController_jobAlertName=&browserTimeZone=Europe/Zurich&_s.crb=bYQhIgqn0MbzwdRaA%2baluxpeS0wy0UARIy5GFiKiaEU%3d</span></a></p><p><a href="https://mathstodon.xyz/tags/FediHire" class="mention hashtag" rel="tag">#<span>FediHire</span></a> <a href="https://mathstodon.xyz/tags/ElectronMicroscopy" class="mention hashtag" rel="tag">#<span>ElectronMicroscopy</span></a> <a href="https://mathstodon.xyz/tags/CryoEM" class="mention hashtag" rel="tag">#<span>CryoEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a></p>
Albert Cardona<p>"DeepFocus: fast focus and astigmatism correction for electron microscopy", Schubert et al. 2024 (Kornfeld lab)</p><p><a href="https://www.nature.com/articles/s41467-024-45042-3" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41467-024</span><span class="invisible">-45042-3</span></a></p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/ElectronMicroscopy" class="mention hashtag" rel="tag">#<span>ElectronMicroscopy</span></a></p>
Albert Cardona<p>A review of the current state of multimodal bioimaging:</p><p>"Integrating cellular electron microscopy with multimodal data to explore biology across space and time", McCafferty et al. 2024 <a href="https://www.cell.com/cell/fulltext/S0092-8674(24)00007-2" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">cell.com/cell/fulltext/S0092-8</span><span class="invisible">674(24)00007-2</span></a></p><p><a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/cryoEM" class="mention hashtag" rel="tag">#<span>cryoEM</span></a> <a href="https://mathstodon.xyz/tags/bioimaging" class="mention hashtag" rel="tag">#<span>bioimaging</span></a></p>
Albert Cardona<p>Postdoc position at the lab of Michael Winding at <a href="https://mathstodon.xyz/tags/TheCrick" class="mention hashtag" rel="tag">#<span>TheCrick</span></a> in London:</p><p><a href="https://crick.wd3.myworkdayjobs.com/External/job/London/Postdoctoral-Fellow---M-Winding-Social-Circuits-and-Connectomics-Laboratory_R1447-1" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">crick.wd3.myworkdayjobs.com/Ex</span><span class="invisible">ternal/job/London/Postdoctoral-Fellow---M-Winding-Social-Circuits-and-Connectomics-Laboratory_R1447-1</span></a></p><p>... on neuronal circuits that drive social interactions between animals.</p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a> <a href="https://mathstodon.xyz/tags/Drosophila" class="mention hashtag" rel="tag">#<span>Drosophila</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/FIBSEM" class="mention hashtag" rel="tag">#<span>FIBSEM</span></a></p>
Albert Cardona<p>"SmartEM: machine-learning guided electron microscopy" by Meirovitch et al 2023 (Nir Shavit and Aravi Samuel labs)<br /><a href="https://www.biorxiv.org/content/10.1101/2023.10.05.561103v1.abstract" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">23.10.05.561103v1.abstract</span></a></p><p>Turn up the imaging rate of your scanning electron microscope by only imaging at high resolution the areas of the image necessary to resolve ambiguities in the low-res image. Motivated by the need for high-throughput but inexpensive imaging for <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a>.</p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/ElectronMicroscopy" class="mention hashtag" rel="tag">#<span>ElectronMicroscopy</span></a></p>
Gaspar Jekely<p>Our group has several 3y+ <a href="https://biologists.social/tags/postdoc" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>postdoc</span></a> openings at the Centre for Organismal Studies at Heidelberg University. Come and join us in Heidelberg to study eye evolution, <a href="https://biologists.social/tags/cilia" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>cilia</span></a>, <a href="https://biologists.social/tags/behaviour" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>behaviour</span></a> by <a href="https://biologists.social/tags/connetomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>connetomics</span></a> <a href="https://biologists.social/tags/vEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEM</span></a> etc. <a href="https://biologists.social/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>neuroscience</span></a> <a href="https://biologists.social/tags/Evolution" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Evolution</span></a> <a href="https://biologists.social/tags/job" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>job</span></a> <a href="https://biologists.social/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>science</span></a> <br><a href="https://adb.zuv.uni-heidelberg.de/info/INFO_FDB$.startup?MODUL=LS&M1=1&M2=0&M3=0&PRO=34018" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">adb.zuv.uni-heidelberg.de/info</span><span class="invisible">/INFO_FDB$.startup?MODUL=LS&M1=1&M2=0&M3=0&PRO=34018</span></a></p>
Albert Cardona<p>A connectome of the optic lobe of the extremely tiny fairy wasp, Megaphragma sp.</p><p>"A complete reconstruction of the early visual system of an adult insect", by Chua et al. 2023 (Chklovskii & Polilov) <a href="https://www.sciencedirect.com/science/article/pii/S096098222301237X" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">sciencedirect.com/science/arti</span><span class="invisible">cle/pii/S096098222301237X</span></a></p><p>Don't miss the supplemental figures.</p><p>"Compared with the honeybee and the fruit fly, Megaphragma exhibits the following miniaturization-related adaptations: a significant reduction in the number of ommatidia, absence of several cell types, reduced size, and denucleation of neurons. Interestingly, the reduction in lens diameter is less than that expected from the optimization of the optical resolution of the eye. This suggests that light sensitivity is a more important<br />consideration when lens diameter approaches the wavelength of light. The absence of wide-field (or non-columnar) lamina neurons in Megaphragma could be a consequence of the smaller number of ommatidia, their larger acceptance angle, and the lower resolving power of the eye."</p><p>Volume assembled with <a href="https://mathstodon.xyz/tags/FijiSc" class="mention hashtag" rel="tag">#<span>FijiSc</span></a> and <a href="https://mathstodon.xyz/tags/TrakEM2" class="mention hashtag" rel="tag">#<span>TrakEM2</span></a>, and its neurons and synapses mapped with <a href="https://mathstodon.xyz/tags/CATMAID" class="mention hashtag" rel="tag">#<span>CATMAID</span></a>. Woohoo!</p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/insects" class="mention hashtag" rel="tag">#<span>insects</span></a> <a href="https://mathstodon.xyz/tags/miniaturization" class="mention hashtag" rel="tag">#<span>miniaturization</span></a></p>
FocalPlane<p>At the end of July, the volume EM community met for their first GRC meeting. Following on from the fantastic coverage at <a href="https://biologists.social/tags/vEMtura23" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEMtura23</span></a>, Ana Correia and James Thompson share their meeting report on FocalPlane.<br>Check it out here: <a href="https://focalplane.biologists.com/2023/08/24/unveiling-the-marvels-of-the-first-ever-gordon-research-conference-on-volume-electron-microscopy-capturing-subcellular-architecture-from-organelles-to-organisms-by-ana-correia-and-james-thompson/" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://</span><span class="ellipsis">focalplane.biologists.com/2023</span><span class="invisible">/08/24/unveiling-the-marvels-of-the-first-ever-gordon-research-conference-on-volume-electron-microscopy-capturing-subcellular-architecture-from-organelles-to-organisms-by-ana-correia-and-james-thompson/</span></a></p><p><a href="https://biologists.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a> <a href="https://biologists.social/tags/biology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>biology</span></a> <a href="https://biologists.social/tags/vEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>vEM</span></a></p>
Albert Cardona<p>Following from Collinson's paper, here is my take on scaling up volume electron microscopy for connectomics in the <a href="https://mathstodon.xyz/tags/UK" class="mention hashtag" rel="tag">#<span>UK</span></a> or any country willing to commit about 10 to 20 million a year:</p><p>"Growing and nurturing a research base in connectomics"<br /><a href="https://albert.rierol.net/tell/20230823_connectomics_research_base.html" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">albert.rierol.net/tell/2023082</span><span class="invisible">3_connectomics_research_base.html</span></a></p><p>From the humble <a href="https://mathstodon.xyz/tags/Drosophila" class="mention hashtag" rel="tag">#<span>Drosophila</span></a> to the <a href="https://mathstodon.xyz/tags/mouse" class="mention hashtag" rel="tag">#<span>mouse</span></a> brain, passing through the mosquito, honeybee, gecko lizards and the Etruscan shrew.</p><p>It's possible, it's feasible, it's timely: therefore we must.</p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a></p>
Albert Cardona<p>"Volume EM: a quiet revolution takes shape" – a review and commentary by Lucy Collinson et al. 2023 on present and future electron microscopy technology and its application <a href="https://www.nature.com/articles/s41592-023-01861-8" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41592-023</span><span class="invisible">-01861-8</span></a></p><p><a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a> <a href="https://mathstodon.xyz/tags/ElectronMicroscopy" class="mention hashtag" rel="tag">#<span>ElectronMicroscopy</span></a></p>
Albert Cardona<p><span class="h-card" translate="no"><a href="https://qoto.org/@cyrilpedia" class="u-url mention">@<span>cyrilpedia</span></a></span> <span class="h-card" translate="no"><a href="https://fediscience.org/@PLOSBiology" class="u-url mention">@<span>PLOSBiology</span></a></span> </p><p>Amusing that the piece includes optical microscopy methods unrelated to neural function, such as STED, PALM, expansion microscopy and CLARITY, but ignores electron microscopy methods key to the current boom in <a href="https://mathstodon.xyz/tags/connectomics" class="mention hashtag" rel="tag">#<span>connectomics</span></a>. In particular, Denk and Horstmann 2004 for SBEM <a href="https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0020329" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">journals.plos.org/plosbiology/</span><span class="invisible">article?id=10.1371/journal.pbio.0020329</span></a> , Knott et al. 2008 for first application of FIBSEM to neuronal tissue <a href="https://www.jneurosci.org/content/28/12/2959" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">jneurosci.org/content/28/12/29</span><span class="invisible">59</span></a> , Schalek et al. 2011 for the ATUM serial section tape-collecting system <a href="https://academic.oup.com/mam/article-abstract/17/S2/966/6947317" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="ellipsis">academic.oup.com/mam/article-a</span><span class="invisible">bstract/17/S2/966/6947317</span></a> , Xu et al. 2017 for e-FIBSEM and Graham et al. 2019 for GridTape TEM <a href="https://www.biorxiv.org/content/10.1101/657346.abstract" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/65</span><span class="invisible">7346.abstract</span></a></p><p><a href="https://mathstodon.xyz/tags/neuroscience" class="mention hashtag" rel="tag">#<span>neuroscience</span></a> <a href="https://mathstodon.xyz/tags/microscopy" class="mention hashtag" rel="tag">#<span>microscopy</span></a> <a href="https://mathstodon.xyz/tags/VolumeEM" class="mention hashtag" rel="tag">#<span>VolumeEM</span></a> <a href="https://mathstodon.xyz/tags/vEM" class="mention hashtag" rel="tag">#<span>vEM</span></a></p>
RTN<p>"Weshalb haben sich Erde und Venus, zwei Planeten, die in Bezug auf Größe und Masse sehr ähnlich sind, über 4,5 Milliarden Jahre so unterschiedlich entwickelt? Diesen und weiteren Fragen will die NASA-Mission VERITAS auf den Grund gehen. Das DLR ist dabei ein wichtiger Partner. .. Pressemitteilung ..DLR."</p><p><a href="https://www.raumfahrer.net/vulkaninsel-island-ist-testumgebung-fuer-nasa-mission-veritas-zur-venus/" rel="nofollow noopener noreferrer" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">raumfahrer.net/vulkaninsel-isl</span><span class="invisible">and-ist-testumgebung-fuer-nasa-mission-veritas-zur-venus/</span></a></p><p><a href="https://chaos.social/tags/DLR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DLR</span></a> <a href="https://chaos.social/tags/EnVision" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>EnVision</span></a> <a href="https://chaos.social/tags/FSAR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>FSAR</span></a> <a href="https://chaos.social/tags/Island" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Island</span></a> <a href="https://chaos.social/tags/JPL" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>JPL</span></a> <a href="https://chaos.social/tags/Lava" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Lava</span></a> <a href="https://chaos.social/tags/Magellan" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Magellan</span></a> <a href="https://chaos.social/tags/NASA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>NASA</span></a> <a href="https://chaos.social/tags/PSL" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>PSL</span></a> <a href="https://chaos.social/tags/Radar" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Radar</span></a> <a href="https://chaos.social/tags/Raumfahrt" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Raumfahrt</span></a> <a href="https://chaos.social/tags/Raumsonde" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Raumsonde</span></a> <a href="https://chaos.social/tags/SAR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>SAR</span></a> <a href="https://chaos.social/tags/VEMulator" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>VEMulator</span></a> <a href="https://chaos.social/tags/VEM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>VEM</span></a> <a href="https://chaos.social/tags/Venus" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Venus</span></a> <a href="https://chaos.social/tags/VERITAS" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>VERITAS</span></a></p><p>10.8.2023</p>
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