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Teresita Porter 🙋🏻‍♀️BOLDistilled: Comprehensive but compact DNA barcode reference libraries<a href="https://ecoevorxiv.org/repository/view/8991/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://ecoevorxiv.org/repository/view/8991/</a><a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/database" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#database</a> <a href="https://ecoevo.social/tags/biodiversity" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biodiversity</a> <a href="https://ecoevo.social/tags/speciesIdentification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#speciesIdentification</a> <a href="https://ecoevo.social/tags/CentreForBiodiversityGenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#CentreForBiodiversityGenomics</a>

Teresita Porter 🙋🏻‍♀️Population genetic data (COI, ddRAD) of Sialis lutaria (Insecta, Megaloptera) from the Emscher catchment (Germany)<a href="https://bdj.pensoft.net/article/141997/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://bdj.pensoft.net/article/141997/</a><a href="https://ecoevo.social/tags/COI" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#COI</a> <a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/ddRAD" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#ddRAD</a> <a href="https://ecoevo.social/tags/PopulationGenetics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#PopulationGenetics</a>

Teresita Porter 🙋🏻‍♀️Use of massive DNA barcoding to monitor biodiversity: a test on forest soil fauna<a href="https://www.biorxiv.org/content/10.1101/2025.02.07.636998v1" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.biorxiv.org/content/10.1101/2025.02.07.636998v1</a><a href="https://ecoevo.social/tags/megabarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#megabarcoding</a> <a href="https://ecoevo.social/tags/soil" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#soil</a> <a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#nanopore</a> <a href="https://ecoevo.social/tags/biodiversity" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biodiversity</a>

Teresita Porter 🙋🏻‍♀️Marine ecozones represent a major gap in Canadian DNA barcoding effortsComparison of the DNA barcode coverage from Canadian terrestrial and marine ecozones.<a href="https://www.facetsjournal.com/doi/full/10.1139/facets-2024-0015" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.facetsjournal.com/doi/full/10.1139/facets-2024-0015</a><a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/Canada" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Canada</a> <a href="https://ecoevo.social/tags/terrestrial" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#terrestrial</a> <a href="https://ecoevo.social/tags/marine" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#marine</a> <a href="https://ecoevo.social/tags/ecozone" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#ecozone</a>

michael17-Oct-2024 Found hundreds of species using <a href="https://mastodon.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> They may not be charismatic megafauna, but they're important nonetheless<a href="https://www.eurekalert.org/news-releases/1061468" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.eurekalert.org/news-releases/1061468</a> <a href="https://mastodon.social/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#science</a> <a href="https://mastodon.social/tags/biodiversity" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biodiversity</a>

Teresita Porter 🙋🏻‍♀️A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project<a href="https://wellcomeopenresearch.org/articles/9-339/v1" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://wellcomeopenresearch.org/articles/9-339/v1</a><a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/DarwinTreeOfLifeProject" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DarwinTreeOfLifeProject</a> <a href="https://ecoevo.social/tags/Eukayotes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Eukayotes</a> <a href="https://ecoevo.social/tags/Britain" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Britain</a> <a href="https://ecoevo.social/tags/Ireland" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Ireland</a>

Donald Hobern<a href="https://mastodon.social/@gvwilson" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@gvwilson</a> Really nice profile of <a href="https://scicomm.xyz/tags/PaulHebert" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#PaulHebert</a> and <a href="https://scicomm.xyz/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> ...There is so much we can never otherwise learn about <a href="https://scicomm.xyz/tags/biodiversity" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biodiversity</a> using non-DNA approaches. Nothing else stands a chance of scaling for invertebrates and fungi.Thanks to the team in <a href="https://scicomm.xyz/tags/Guelph" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Guelph</a>, here is a tiny part of what this technology revealed about my local fauna in Canberra:<a href="https://stangeia.hobern.net/lepidoptera-barcoding-in-araba-bioscan/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://stangeia.hobern.net/lepidoptera-barcoding-in-araba-bioscan/</a>

Teresita Porter 🙋🏻‍♀️A universal DNA barcode for the Tree of Life<a href="https://ecoevorxiv.org/repository/view/6567/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://ecoevorxiv.org/repository/view/6567/</a>"a novel barcoding method that uses exceptionally low-coverage genome skim data to create a “varKode”, a two-dimensional image representing the genomic landscape of a species. Using these varKodes, we then train neural networks for precise taxonomic identification."<a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/varKode" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#varKode</a> <a href="https://ecoevo.social/tags/genomeSkimming" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#genomeSkimming</a> <a href="https://ecoevo.social/tags/preprint" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#preprint</a>

Teresita Porter 🙋🏻‍♀️**A practical workflow for forensic species identification using direct sequencing of real-time PCR products**<a href="https://link.springer.com/article/10.1007/s11033-023-08980-7" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://link.springer.com/article/10.1007/s11033-023-08980-7</a><a href="https://ecoevo.social/tags/qPCR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#qPCR</a> <a href="https://ecoevo.social/tags/rtPCR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#rtPCR</a> <a href="https://ecoevo.social/tags/sequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#sequencing</a> <a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/forensics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#forensics</a>

rspI need to be able to do DNA metabarcoding for diet analysis cheaply. Can't afford >$6,000 for 100 samples.<a href="https://ecoevo.social/tags/DNAmetaBarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAmetaBarcoding</a> <a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a>

Teresita Porter 🙋🏻‍♀️"Rapid species discovery and identification with real-time barcoding facilitated by ONTbarcoder 2.0 and Oxford Nanopore R10.4"<a href="https://www.biorxiv.org/content/10.1101/2023.06.26.546538v2" rel="nofollow noopener noreferrer" target="_blank">https://www.biorxiv.org/content/10.1101/2023.06.26.546538v2</a>The accompanying video is cool <a href="https://twitter.com/RudolfMeier15/status/1674801657287413760?s=20" rel="nofollow noopener noreferrer" target="_blank">https://twitter.com/RudolfMeier15/status/1674801657287413760?s=20</a><a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#nanopore</a> <a href="https://ecoevo.social/tags/GUI" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#GUI</a>

Karl MagnaccaHas anyone worked with sequences from BOLD and found...anomalies? I've been working with a big batch and just discovered that 1/3 of them have inexplicable deletions in the text sequence, even though the chromatograms show it's unambiguously complete. Seems like a problem... <a href="https://ecoevo.social/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a> <a href="https://ecoevo.social/tags/DNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNA</a>

Donald HobernI've updated the data mirror and browser interface I host for exploring Australian public <a href="https://scicomm.xyz/tags/DNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNA</a> <a href="https://scicomm.xyz/tags/barcode" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#barcode</a> records from <a href="https://scicomm.xyz/tags/BOLD" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#BOLD</a>. I wanted an easier way to visualise and break down the relationships between the scientific names provided as identifications and the signals from the barcode BIN clusters.<a href="https://bold-au.hobern.net/" rel="nofollow noopener noreferrer" target="_blank">https://bold-au.hobern.net/</a>The images show specimens identified as Dysbatus stenodesma and the two BINS into which those specimens fall.<a href="https://bold-au.hobern.net/specimens.php?taxonID=6175" rel="nofollow noopener noreferrer" target="_blank">https://bold-au.hobern.net/specimens.php?taxonID=6175</a><a href="https://scicomm.xyz/tags/DnaBarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DnaBarcoding</a> <a href="https://scicomm.xyz/tags/entomology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#entomology</a> <a href="https://scicomm.xyz/tags/Lepidoptera" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Lepidoptera</a>

Donald HobernIntroduction (now with hashtags). I'm a long-term <a href="https://scicomm.xyz/tags/naturalist" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#naturalist</a> and <a href="https://scicomm.xyz/tags/biodiversity" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biodiversity</a> informatician/#datascientist based in Canberra. My focus is on <a href="https://scicomm.xyz/tags/FAIR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#FAIR</a> and open data, local biodiversity monitoring, <a href="https://scicomm.xyz/tags/insect" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#insect</a> diversity, (automated) <a href="https://scicomm.xyz/tags/light" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#light</a>-trapping, species reference lists, <a href="https://scicomm.xyz/tags/DNAbarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DNAbarcoding</a>, with spillover to the subject of my other work responsibilities, crop data management.

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