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Today the peer-reviewed version of our preprint is out:

"The #connectome of an insect brain"
https://www. science.org/doi/10.1126/scienc e.add9330

Congrats to co-first authors Michael Winding and Benjamin Pedigo, and to all our lab members and collaborators who made this work possible over the years. A journey that started over 10 years ago–and yet this is but a new beginning. So much more to come.

See my #tootprint on the preprint from back in the Autumn: https://mathstodon.xyz/@albertcardona/109422190525090990

The data is available both as supplements and directly via #CATMAID thanks to hosting by the #VirtualFlyBrain:
https://l1em.catmaid.virtualflybrain.o rg/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6)

(The "Winding, Pedigo et al. 2023" annotation listing all included neurons will appear very soon in an upcoming update.)

#neuroscience #connectomics #Drosophila #DrosophilaLarva

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Today the peer-reviewed version of our preprint is out:

"The #connectome of an insect brain"
https://www. science.org/doi/10.1126/scienc e.add9330

Congrats to co-first authors Michael Winding and Benjamin Pedigo, and to all our lab members and collaborators who made this work possible over the years. A journey that started over 10 years ago–and yet this is but a new beginning. So much more to come.

See my #tootprint on the preprint from back in the Autumn: https://mathstodon.xyz/@albertcardona/109422190525090990

The data is available both as supplements and directly via #CATMAID thanks to hosting by the #VirtualFlyBrain:
https://l1em.catmaid.virtualflybrain.o rg/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6)

(The "Winding, Pedigo et al. 2023" annotation listing all included neurons will appear very soon in an upcoming update.)

#neuroscience #connectomics #Drosophila #DrosophilaLarva

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The connectome of the Drosophila larval brain.

The morphologies of all brain neurons, reconstructed from a synapse-resolution EM volume, and the synaptic connectivity matrix of an entire brain. This connectivity information was used to hierarchically cluster all brains into 93 cell types, which were internally consistent based on morphology and known function.

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3,013 neurons, half a million synapses: the complete #connectome of the whole #Drosophila larval brain!

Winding, Pedigo et al. 2022. "The connectome of an insect brain" https://www. biorxiv.org/content/10.1101/20 22.11.28.516756v1

We’ve mapped and analysed its circuit architecture, from sensory neurons to brain output neurons, as reconstructed from volume electron microscopy, and here is what we found. 1/

#neuroscience #connectomics #vEM #volumeEM

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3,013 neurons, half a million synapses: the complete #connectome of the whole #Drosophila larval brain!

Winding, Pedigo et al. 2022. "The connectome of an insect brain" https://www. biorxiv.org/content/10.1101/20 22.11.28.516756v1

We’ve mapped and analysed its circuit architecture, from sensory neurons to brain output neurons, as reconstructed from volume electron microscopy, and here is what we found. 1/

#neuroscience #connectomics #vEM #volumeEM

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Rendering of all 3,013 neurons that make up the brain of the larva of the vinegar fly, Drosophila melanogaster.

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#introduction To fill in my profile tags, a thread:

#TrakEM2 open source software mostly for #connectomics, and supports manual and automatically montaging and aligning overlapping 2D image tiles (with #SIFT features and rigid or elastic transformation models), and reconstructing by painting volumes or tracing branched neuronal arbors neurons plus synapses to map a #connectome from #vEM (volume electron microscopy).

See: https://journals.plos.org/plosone/arti cle?id=10.1371/journal.pone.0038011

Git repository at: https://github.com/trakem2/

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#introduction To fill in my profile tags, a thread:

#TrakEM2 open source software mostly for #connectomics, and supports manual and automatically montaging and aligning overlapping 2D image tiles (with #SIFT features and rigid or elastic transformation models), and reconstructing by painting volumes or tracing branched neuronal arbors neurons plus synapses to map a #connectome from #vEM (volume electron microscopy).

See: https://journals.plos.org/plosone/arti cle?id=10.1371/journal.pone.0038011

Git repository at: https://github.com/trakem2/

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Figure 2 of Cardona et al. 2012 PLoS ONE, illustrating neuronal arbors reconstructed using TrakEM2 software.

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The web-based open source software #CATMAID was devised as "google maps but for volumes". Documentation at https://catmaid.org and source code at https://github.com/catmaid/CATMAID/

Modern #CATMAID enables hundreds of #neuroscience researchers world wide to collaboratively map neuronal circuits in large datasets limited only by bandwidth and server-side storage to map and analyse a whole brain #connectome.

See the #Drosophila larval CNS at the #VirtualFlyBrain server: https://l1em.catmaid.virtualflybrain.o rg/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6)

# orderedItems[].contentMap.en
The web-based open source software #CATMAID was devised as "google maps but for volumes". Documentation at https://catmaid.org and source code at https://github.com/catmaid/CATMAID/

Modern #CATMAID enables hundreds of #neuroscience researchers world wide to collaboratively map neuronal circuits in large datasets limited only by bandwidth and server-side storage to map and analyse a whole brain #connectome.

See the #Drosophila larval CNS at the #VirtualFlyBrain server: https://l1em.catmaid.virtualflybrain.o rg/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6)

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Screenshot of CATMAID software illustrating various widgets to analyze neurons and neuronal circuits.